Recombinant aprotinin
Serine protease inhibitor, same sequence as bovine aprotinin.
A competitive inhibitor of trypsin, chymotrypsin and kallikrein with the same amino-acid sequence as aprotinin from bovine lung. It replaces animal-derived aprotinin in biotech processes, for example to stop trypsin digestion.
Chemical identity
- Molecular weight
- 6511 g/mol
- Sequence
- RPDFCLEPPYTGPCKARIIRYFYNAKAGLCQTFVYGGCRAKRNNFKSAEDCMRTCGGA
- InChIKey
- ZPNFWUPYTFPOJU-UHFFFAOYSA-N
- PubChem CID
- 22833874
Specification
| Made by | E. coli expression, recombinant |
| Appearance | Clear, colourless to light yellow liquid |
| Specific activity | ≥ 3.0 EPU/mg protein |
| Absorbance at 277 nm | ≤ 0.8 (3 EPU/mL) |
| Purity (SDS-PAGE) | ≥ 95 % |
| Storage | −20 °C, 24 months |
- Supply at 10 g scale and up.
- No animal-derived materials in the process.